Coding

Part:BBa_M45447:Design

Designed by: Charles Harding   Group: UtahState_BE5930_-_S14   (2015-03-31)


Blue light activated transcription factor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 868
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
    Illegal AgeI site found at 889
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 454
    Illegal BsaI site found at 892
    Illegal SapI.rc site found at 568


Design Notes

From Pseudocercospora fijiensis, backtranslated to human genome with DNA 2.0 Gene Designer. Codon optimized for PstI and SmaI.

Source

http://www.ncbi.nlm.nih.gov/protein/EME83354.1

References

GenBank: EME83354.1